In one such study, the researchers used NimbleGen Sequence Capture 385K arrays to enrich a 1.91 Mb region of the genome containing 95 cancer-associated genes in 6 acute myeloid leukemia (AML) samples. They then sequenced the captured DNA with the GS FLX Titanium series chemistry and analyzed the results with the company ™s GS Reference Mapper software. The results showed, for the first time that point mutations, deletions and insertions, as well as fusion genes from translocations and inversions could be detected in a one-step methodological approach.

Another study presented by Dr. Alexander Kohlmann and Vera Grossmann used ultra-deep sequencing of amplicons to accurately identify mutations in oncogenic regions within 95 samples of leukemia and myeloproliferative neoplasms. Amplicon sequencing with the GS FLX System is a particularly straightforward and powerful method to detect a wide range of molecular mutations with high sensitivity. It is of particular utility for characterizing the constantly growing number of target genes used to distinguish molecular subtypes of hematological malignancies, explained Dr. Alexander Kohlmann, PhD, Head of the NGS group at the MLL. This technology has the potential to immediately change the way we obtain novel molecular insights underlying this disease.

For more information on 454 Sequencing Systems, visit www.454. For more information on Roche Nimblegen Sequence Capture arrays, visit www.nimblegen.

Source Roche